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Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding

Publication

Title of Publication
Mechanically switching single-molecule fluorescence of GFP by unfolding and refolding
Publication Year
2017
Authors
Ganim Z, Rief M.
Journal
Proc Natl Acad Sci U S A
DOI
https://doi.org/10.1073/pnas.1704937114

Construct

Gene Name
GFP
Sequence
MCGGTGGKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFICTTGKLPVPWPTLVTTFSYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLEFVTAAGITHGMDELYCGHHHHHH