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Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein

Title of Publication

Single-molecule chemo-mechanical unfolding reveals multiple transition state barriers in a small single-domain protein

Publication Year

2015

Authors

Guinn EJ, Jagannathan B, Marqusee S.

Journal

Nat Commun

DOI

https://doi.org/10.1038/ncomms7861

Construct Name

R19C/N59C src SH3

Gene Name

src SH3

Sequence

MVTTFVALYDYESRTETDLSFKKGERLQIVNNTEGDWWLAHSLSTGQTGYIPSNYVAPSDSIQAEEWYFGKITRRESERLLLNAENPRGTFLVRESETTKGAYCLSVSDFDNAKGLNVKHYKIRKLDSGGFYITSRTQFNSLQQLVAYYSKHADGLCHRLTTVCPTSKPQTQGLAKDAWEIPRESLRLEVKLGQGCFGEVWMGTWNGTTRVAIKTLKPGTMSPEAFLQEAQVMKKLRHEKLVQLYAVVSEEPIYIVTEYMSKGSLLDFLKGETGKYLRLPQLVDMAAQIASGMAYVERMNYVHRDLRAANILVGENLVCKVADFGLARLIEDNEYTARQGAKFPIKWTAPEAALYGRFTIKSDVWSFGILLTELTTKGRVPYPGMVNREVLDQVERGYRMPCPPECPESLHDLMCQCWRKEPEERPTFEYLQAFLEDYFTSTEPQYQPGENL

UniProt link

https://www.ebi.ac.uk/interpro/entry/InterPro/IPR001452/