SMASH-BIO

The Single Molecule Analysis and Spectroscopy Hub

Go Back
Mechanically tightening, untying and retying a protein trefoil knot by single-molecule force spectroscopy-1

Mechanically tightening, untying and retying a protein trefoil knot by single-molecule force spectroscopy-1

Publication

Title of Publication
Mechanically tightening, untying and retying a protein trefoil knot by single-molecule force spectroscopy
Publication Year
2020
Authors
Wang H, Li H.
Journal
Chem Sci
DOI
https://doi.org/10.1039/d0sc02796k

Construct

Construct Name
TrmD9,246–(NuG2)2
Gene Name
tRNA (guanosine-1) methyltransferase (TrmD)
Sequence
MRGSHHHHHHGSMWIGIISLCPEMFRAITDYGVTGRAVKNGLLSIQSWSPR DFTHDRHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYL SPQGRKLDQAGVSELATNQKLILVSGRYEGIDERVIQTEIDEEWSIGDYVL SGGELPAMTLIDSVSRFIPGVLGHEASATEDSFAEGLLDSPHYTRPEVLEG MEVPPVLLSGNHAEIRRWRLKQSLGRTWLRRPELLENLALTEEQARLLA EFKTEHAQRSMDTYKLVIVLNGTTFTYTTEAVDAATAEKVFKQYANDNGVDGEW TYDDATKTFTVTERSMDTYKLVIVLNGTTFTYTTEAVDAATAEKVFKQYANDNGVD GEWTYDDATKTFTVTERSGGTKC
PDB ID
1P9P
UniProt link
https://www.uniprot.org/uniprotkb/P0A873/entry