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Optical trapping with high forces reveals unexpected behaviors of prion fibrils

Optical trapping with high forces reveals unexpected behaviors of prion fibrils

Publication

Title of Publication
Optical trapping with high forces reveals unexpected behaviors of prion fibrils
Publication Year
2010
Authors
Dong J, Castro CE, Boyce MC, Lang MJ, Lindquist S.
Journal
Nat Struct Mol Biol
DOI
https://doi.org/10.1038/nsmb.1954

Construct

Gene Name
Sup35 prion NM domains w/ mutant
Sequence
"MSDSNQGNNQQNYQQYSQNGNQQQGNNRYQGYQAYNAQAQPAGGYYQNYQGYSGYQQGGYQQYNPDAGYQ QQYNPQGGYQQYNPQGGYQQQFNPQGGRGNYKNFNYNNNLQGYQAGFQPQSQGMSLNDFQKQQKQAAPKP KKTLKLVSSSGIKLANATKKVGTKPAESDKKEEEKSAETKEPTKEPTKVEEPVKKEEKPVQTEEKTEEKS ELPKVEDLKISESTHNTNNANVTSADALIKEQEEEVDDEVVNDMFGGKDHVSLIFMGHVDAGKSTMGGNL LYLTGSVDKRTIEKYEREAKDAGRQGWYLSWVMDTNKEERNDGKTIEVGKAYFETEKRRYTILDAPGHKM YVSEMIGGASQADVGVLVISARKGEYETGFERGGQTREHALLAKTQGVNKMVVVVNKMDDPTVNWSKERY DQCVSNVSNFLRAIGYNIKTDVVFMPVSGYSGANLKDHVDPKECPWYTGPTLLEYLDTMNHVDRHINAPF MLPIAAKMKDLGTIVEGKIESGHIKKGQSTLLMPNKTAVEIQNIYNETENEVDMAMCGEQVKLRIKGVEE EDISPGFVLTSPKNPIKSVTKFVAQIAIVELKSIIAAGFSCVMHVHTAIEEVHIVKLLHKLEKGTNRKSK KPPAFAKKGMKVIAVLETEAPVCVETYQDYPQLGRFTLRDQGTTIAIGKIVKIAE"
Mutations
184Cysteine
UniProt link
https://www.uniprot.org/uniprotkb/P05453/entry